Injury in Intact Leaves, as Detected by Chlorophyll Fluorescence

Z. Naturforsch. 43c, 269-274 (1988); received December 7, 1987 Chlorophyll Fluorescence, S02 , Spinach, Intact Leaves, Calvin Cycle The effects of short-time fumigation (0-60 min) of intact spinach leaves with S02 (2 ppm) on the photosynthetic apparatus were investigated. A rather high S02 concentration was applied to monitor immediate effects on the fluorescence behaviour with the influence of repair processes or secondary types of damage being minimized. Three different types of in vivo chlorophyll fluorescence measurements were used: Rapid induction kinetics (Kautsky effect), slow induction kinetics with repetitive application of saturation pulses (saturation pulse method), and decay kinetics following a single turnover saturating flash. The slow induction kinetics with repetitive application of saturation pulses reacts in the most sensitive way indicating a primary damage at the level of the enzymatic reactions of the Calvin cycle. It is suggested that stromal acidification upon S02 uptake interferes with light activation of Calvin cycle enzymes. With longer fumigation times also damage at the level of photosystem II becomes apparent: A decrease in variable fluorescence yield reflects a lowering of photosystem II quantum yield, and the slowing down of fluorescence relaxation kinetics reveals an effect on the secondary electron transport from QA to QB. The detrimental effects of S02 depend to a great extent on the application of light during fumigation. Besides a light requirement for S02 uptake by stomata opening also the possibility of photoinhibitory damage is discussed. The susceptibility of leaves to photoinhibition may increase with a lowering of Calvin cycle activity by S02.